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NEN Life Science methyl-1,2–3h]dttp net520a
Methyl 1,2–3h]Dttp Net520a, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methyl-3h%5Ddttp/0+25+%CE%BCmole++methyl+1+2+3h+dttp+net520a/pmc07644855-49-38-42
Average 90 stars, based on 1 article reviews
methyl-1,2–3h]dttp net520a - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Dicaffeoylquinic and Dicaffeoyltartaric Acids Are Selective Inhibitors of Human Immunodeficiency Virus Type 1 Integrase
Article Snippet: RT activities in culture supernatant fluids were determined by the method of Poiesz et al., with poly(rA-dT)15 as a template primer and 25 mCi of [methyl-3H]dTTP per reaction (specific activity, 80.4 Ci/ mmol; NEN) (41).

Article Title: Identification of two apurinic/apyrimidinic endonucleases from Caenorhabditis elegans by cross-species complementation.
Article Snippet: The Saccharomyces cerevisiae mutant strain YW778, which lacks apurinic/apyrimidinic (AP) endonuclease and 3′-diesterase DNA repair activities, displays high levels of spontaneous mutations and hypersensitivities to several DNA damaging agents.. We searched a cDNA library derived from the nematode Caenorhabditis elegans for gene products that would rescue the DNA repair defects of this yeast mutant.. We isolated two genes, apn-1 and exo-3, encoding proteins that have not been previously characterized.

Article Title: Retinoblastoma: Magnetic Isotope Effects Might Make a Difference in the Current Anti-Cancer Research Strategy.
Article Snippet: For the DNApolβ catalytic activity measurements, an incorporation of (Methyl-1,2-3H)dTTP (90–120 Ci/mmol, NET520A, NEN) into the nascent enzyme-produced DNA sequences was detected to be expressed then as (3H)cpmDNA/ mg protein.

Article Title: Functional characterization of the Caenorhabditis elegans DNA repair enzyme APN-1.
Article Snippet: Caenorhabditis elegans possesses two distinct DNA repair enzymes EXO-3 and APN-1 that have been identified by cross-specie complementation analysis of the Saccharomyces cerevisiae apn1 apn2 tpp1 triple mutant deficient in the ability to repair apurinic/apyrimidinc (AP) sites and DNA strand breaks with blocked 3′-ends.. While purified EXO-3 directly incises AP sites and removes 3′-blocking groups, such characterization has not been previously reported for APN-1.. We recently documented that C. elegans knockdown for apn-1 is unable to maintain integrity of the genome.

Article Title: Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage.
Article Snippet: The labeled [methyl-3H]dTTP (NET221X from NEN Life Science Products; 37.0 MBq) was added to the reaction mixture to a specific activity of 1260 cpm/pmol.

Activity Assay:

Article Title: Dicaffeoylquinic and Dicaffeoyltartaric Acids Are Selective Inhibitors of Human Immunodeficiency Virus Type 1 Integrase
Article Snippet: RT activities in culture supernatant fluids were determined by the method of Poiesz et al., with poly(rA-dT)15 as a template primer and 25 mCi of [methyl-3H]dTTP per reaction (specific activity, 80.4 Ci/ mmol; NEN) (41).

Article Title: Identification of two apurinic/apyrimidinic endonucleases from Caenorhabditis elegans by cross-species complementation.
Article Snippet: The Saccharomyces cerevisiae mutant strain YW778, which lacks apurinic/apyrimidinic (AP) endonuclease and 3′-diesterase DNA repair activities, displays high levels of spontaneous mutations and hypersensitivities to several DNA damaging agents.. We searched a cDNA library derived from the nematode Caenorhabditis elegans for gene products that would rescue the DNA repair defects of this yeast mutant.. We isolated two genes, apn-1 and exo-3, encoding proteins that have not been previously characterized.

Article Title: Retinoblastoma: Magnetic Isotope Effects Might Make a Difference in the Current Anti-Cancer Research Strategy.
Article Snippet: For the DNApolβ catalytic activity measurements, an incorporation of (Methyl-1,2-3H)dTTP (90–120 Ci/mmol, NET520A, NEN) into the nascent enzyme-produced DNA sequences was detected to be expressed then as (3H)cpmDNA/ mg protein.

Article Title: Functional characterization of the Caenorhabditis elegans DNA repair enzyme APN-1.
Article Snippet: Caenorhabditis elegans possesses two distinct DNA repair enzymes EXO-3 and APN-1 that have been identified by cross-specie complementation analysis of the Saccharomyces cerevisiae apn1 apn2 tpp1 triple mutant deficient in the ability to repair apurinic/apyrimidinc (AP) sites and DNA strand breaks with blocked 3′-ends.. While purified EXO-3 directly incises AP sites and removes 3′-blocking groups, such characterization has not been previously reported for APN-1.. We recently documented that C. elegans knockdown for apn-1 is unable to maintain integrity of the genome.

Article Title: Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage.
Article Snippet: The labeled [methyl-3H]dTTP (NET221X from NEN Life Science Products; 37.0 MBq) was added to the reaction mixture to a specific activity of 1260 cpm/pmol.



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